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pLS207 [981]

規格:

價格:

編號:B217526

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLS207 [981]

商品貨號

B217526

Designations

pLS207 [981]

GenBank Number

M14340

培養基

Species

Streptococcus pneumoniae (Klein) Chester

Depositors

Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory

Vector

Construct size (kb): 9.899999618530273

Insert

DNA: genomic

Insert lengths(kb): 3.5

Gene product: [dpnC]

Insert Size (kb)

3.5

Media

ATCC^® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains NcoI, XmnI (2), and PstI sites.

Strain is noncapsulated and never reverts to encapsulated form.

Should be treated as a class II biohazard.

A 1.7 kb EcoRV/PstI fragment terminating at the PstI site near the distal end of the insert contains the DpnI-specific gene. The ATG for dpnC begins at nucleotide 227 from the EcoRV site and encodes a 30 kDa protein.

The dpnD coding sequence, transcribed in the same orientation as dpnC, extend from approximately 1050 to 1500 bp from the EcoRV site.

According to the published sequence, the limits of the genes are as follows: dpnC from 526 to 1041 and dpnD from 1038 to 1499.

References

Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990

Lacks SA, et al. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intracellular cassette mechanism. Cell 46: 993-1000, 1986. PubMed: 3019562

Sanford A Lacks, personal communication

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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