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lambdaSK22 [clone 1]
lambdaSK22 [clone 1]
規(guī)格:
價(jià)格:
編號:B217559
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
lambdaSK22 [clone 1]
商品貨號
B217559
Designations
lambdaSK22 [clone 1]
Depositors
ER Zabarovsky, G Winberg, G Klein
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 47.7999992370605500
Vector: lambdaSK22 (phage, lambda - replacement)
Marker(s):red+ gam+
Construct size (kb): 47.79999923706055
Features: insert detection: Spi+
marker(s): red+ gam+
replicon: lambda, pMB1, M13
Applications
vector containing primer sites useful for sequencing
vector for constructing genomic libraries
vector for other uses
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5, 9.5; EcoRI--25.0, 13.5, 9.5; SalI--20.0, 9.5, 9.2, 4.2, 3.9.
Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in.
The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI.
The restriction sites to be used for conversion to phagemid are: ClaI BspMII SalI.
The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7.
The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/XbaI - ClaI SalI - arm.
References

Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275

Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992.

Shipped
freeze-dried
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