Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
MicroRNAs (miRNAs) are a new class of small noncoding RNAs that post-transcriptionally regulate the expression of target mRNA transcripts. Dicer is an RNase III family endoribonuclease that has the biofunction of processing miRNAs. Loss of Dicer1 leads to a dramatic decrease in levels of cellular microRNA, allowing for investigations into the roles of microRNAs in various cellular functions.
Dicer 1f/f (ATCC® CRL-3220™) is the wild-type control for Dicer 1-/- (ATCC® CRL-3221™), Dicer deficient mesenchymal stem cells. Dicer is an RNase III family endoribonuclease tumor suppressor that processes miRNAs. It has been suggested that complete Dicer 1 loss and the subsequent misregulation of gene expression can inhibit tumor growth rates through reduced proliferation and increased cell death. This suggests that the targeted inhibition of miRNA pathway elements, particularly Dicer I, may be a potential therapy for the treatment of cancer.
Ravi A, et al. Proliferation and tumorigenesis of a murine sarcoma cell line in the absence of DICER1. Cancer Cell 21(6): 848-855, 2012. PubMed: 22698408
Gurtan AM, et al. In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. RNA 18: 1116-1122, 2012. PubMed: 22546613
