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pT24-C3
pT24-C3
規格:
貨期:
編號:B218156
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pT24-C3
商品貨號
B218156
Designations
pT24-C3
GenBank Number

J00277

Species
Homo sapiens, human
Depositors
M Barbacid
Vector
Construct size (kb): 10.89999961853027
Insert
DNA: genomic
Insert lengths(kb): 6.599999904632568
Tissue: bladder carcinoma T24 cell line
Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]
Alleles: D1, D2
Insert Size (kb)
6.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.3; SacI--7.0, 3.0, 0.9.
Shows transforming activity; insert contains 3 SacI sites (major fragments 3.0, 2.7, 0.85 kb), as well as XhoI, PvuII, XbaI, KpnI, SphI, ClaI, BglII sites; plasmid cut with SacI gives 7.0, 3.0, 0.85 kb fragments.
This probe contains the transforming form of the HRAS1 gene mutated by a G to T transversion at the 12th codon; it also contains 3' and 5' flanking sequences.
References

Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3' to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828

Pulciani S, et al. Oncogenes in human tumor cell lines: Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc. Natl. Acad. Sci. USA 79: 2845-2849, 1982. PubMed: 6953433

Santos E, et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298: 343-347, 1982. PubMed: 6283384

Reddy EP. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science 220: 1061-1063, 1983. PubMed: 6844927

Pulciani S, et al. Transforming genes in human tumors. J. Cell. Biochem. 20: 51-61, 1982. PubMed: 6761348

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