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pLamB1L
pLamB1L
規格:
價格:
編號:B218282
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLamB1L
商品貨號
B218282
Designations
pLamB1L
GenBank Number

M15525

培養基
Species
Mus musculus, mouse
Depositors
I Oberbaumer
Applications
A HindIII/SphI digestion produces fragments of 1.630 kb (without tails) and 0.855 kb (with a GC tail).
Vector
Construct size (kb): 5.349999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.654999971389771
Tissue: teratocarcinoma PYS-2/12 cell line
Gene product: laminin, B1 polypeptide [Lamb1]
Alleles: a, l
Insert Size (kb)
2.655
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 2.6; EcoRI--5.4; HindIII--2.9, 2.5; KpnI--5.4; SalI--5.4.
The insert includes the following restriction sites (approximate kb from the 5' end): PvuII--0.12, 0.76, 1.38, 1.45, 2.23; ApaI--0.78; PstI--0.88, 1.40, 1.78, 2.3; SacI--0.89, 1.39; BglII--1.61; HindIII--2.48.
A HindIII/SphI digestion produces fragments of 1.630 kb (without tails) and 0.855 kb (with a GC tail). The XbaI site at nt 4730 of the reported sequence does not cut.
Extends from nt 2641 to 5296 of the reported sequence, encoding part of domains I and III and all of domains II and alpha.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Sasaki M, et al. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc. Natl. Acad. Sci. USA 84: 935-939, 1987. PubMed: 3493487

Ilse Oberbaumer, personal communication

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