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pV-mos
pV-mos
規格:
價格:
編號:B218292
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pV-mos
商品貨號
B218292
Designations
pV-mos
GenBank Number

J02266

培養基
Species
Moloney murine sarcoma virus, Moloney murine sarcoma virus
Depositors
SA Aaronson, SR Tronick
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: Moloney murine sarcoma viral (v-mos) oncogene homolog [MOS]
Alleles: B2, B1
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The BglI site on the insert end was replaced by a HindIII linker before cloning.
The insert is the BglI/HindIII fragment from 4116 to 5021 on the Mo-MSV map. The insert contains KpnI, HpaI, SstII, PstI (4), HpaII (5), DdeI (3), and HinfI (4) sites. The insert excludes v-mos helper viral junctions.
Classification
Retroviridae, Gammaretrovirus, Mammalian virus group
References

Reddy EP, et al. Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome. Science 214: 445-450, 1981. PubMed: 6170110

Tronick SR, et al. Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. Proc. Natl. Acad. Sci. USA 76: 6314-6318, 1979. PubMed: 392518

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