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pPAI-E5.2

規格:

價格:

編號:B218333

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pPAI-E5.2

商品貨號

B218333

Designations

pPAI-E5.2

GenBank Number

J03764

培養基

Species

Homo sapiens, human

Depositors

PJ Bosma

Applications

There are 4 putative polyadenylation signals between nucleotides 12092 and 12141. There is also an AATAAT sequence at 11174 surrounded by an AT-rich region which could serve as a polyadenylation signal.

Vector

Construct size (kb): 7.900000095367432

Insert

DNA: genomic

Insert lengths(kb): 5.199999809265137

Gene product: plasminogen activator inhibitor, type I [PAI1]

Insert Size (kb)

5.200

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Among the restriction sites in the insert are the following: PstI (4), BglI (2), BamHI (2), NcoI (1), AatII (1), BstEII (1), and XbaI (1).

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 2.7; PstI--3.3, 2.9, 0.98, 0.48, 0.24; BamHI--5.4, 1.4, 0.87; SalI--7.9; BglI--5.3, 1.3, 1.08.

Insert begins in intron 7 and extends about 2.2 kb 3' to the last exon, exon 9. The insert contains multiple Alu repeats and two purine-rich sequences.

References

Bosma PJ, et al. Human plasminogen activator inhibitor-1 gene. Promoter and structural gene nucleotide sequences. J. Biol. Chem. 263: 9129-9141, 1988. PubMed: 3132455

B179243：pPAI-E5.2

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