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BJ-5ta
BJ-5ta
規格:
價格:
編號:B218466
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BJ-5ta
商品貨號
B218466
Organism
Homo sapiens, human
Tissue
Foreskin
培養基
Cell Type
Fibroblast immortalized with hTERT
Product Format
frozen
Morphology
Fibroblast-like
Culture Properties
Adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Normal
Age
Neonatal
Gender
Male
Storage Conditions
Liquid nitrogen vapor phase
Karyotype
This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal.
Images
Derivation

The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332]. 

Antigen Expression

Antigen expression: Positive for fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.).

Negative for the pan-cytokeratin epithelial marker; Homo sapiens (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody).

Complete Growth Medium
A 4:1 mixture of Dulbecco's medium and Medium 199 with supplements as follows :
4 parts of Dulbecco's Modified Eagle's Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate
1 part of Medium 199
Supplemented with:
0.01 mg/ml hygromycin B
10% fetal bovine serum
Subculturing
Volumes are given for a 75 cm2 flask. Recommended use of Corning® T-75 flasks (catalog #430641). Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Add 3.0 to 5.0 mL of 0.25% trypsin-0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes).
    3. Note: To avoid clumping do not hit or shake the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 10.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  4. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 3 x 103 to 5 x 103 viable cells/cm2 is recommended. Maintain cultures at a cell concentration between 8 x 103 and 1 x 104 cells/cm2.
  5. Subcultivation ratio: 1:2 to 1:3 twice weekly
  6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:3 twice weekly
Medium Renewal: every 2 to 3 days
Note: Subculture when cell concentration reaches between 8 X 103 and 1 X 104 cells/cm2.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation
Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,9
D16S539: 9,13
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 10,11
vWA: 16,18
Population Doubling Level (PDL)
As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor
Geron Corporation
Deposited As
Homo sapiens
References

Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332

Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802

Aiastui A, et al. Salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells. Infect Immun 78(6):2700-13, 2010. PubMed: 20368348

Janeckova L, et al. HIC1?Tumor?Suppressor?Loss?Potentiates?TLR2/NF-κB?Signaling?and?Promotes?Tissue?Damage-Associated?Tumorigenesis. Mol Cancer Res?13(7):1139-48, 2015. PubMed: 25934696

Amadori S, et al. Effect of sterilization and crosslinking on gelatin films. J Mater Sci Mater Med 26(2):69, 2015. PubMed: 25631265

La Gatta A, et al. Hyaluronan dermal fillers via crosslinking with 1,4-butandiol diglycidyl ether: Exploitation of heterogeneous reaction conditions. J Biomed Mater Res B Appl Biomater 104(1):9-18, 2016. PubMed: 25611588

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