Differs from pBR322 in that the EcoRI site is replaced with a unique SmaI site flanked by EcoRI sites.
A general purpose plasmid vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Prentki P, Krisch HM. A modified pBR322 vector with improved properties for the cloning, recovery, and sequencing of blunt-ended DNA fragments. Gene 17: 189-196, 1982. PubMed: 6282713
