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lambdaepsilon10EZZ

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編號:B218587

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

lambdaepsilon10EZZ

商品貨號

B218587

Designations

lambdaepsilon10EZZ

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 44.0

Insert

DNA: genomic

Insert lengths(kb): 14.39999961853027

Tissue: B lymphocyte (EBV transformed)

Gene product: immunoglobulin epsilon [IGHE]

Insert Size (kb)

14.400

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.8; SacI--20.0, 9.2, 3.8, 2.9, 2.3, 1.8, 1.2, 1.0, 0.65, 0.58, 0.52; XhoI--20.0, 14.0, 9.2;

SmaI--20.0, 5.8, 3.7, 3.5, 1.25, 0.90, 0.84, 0.76; PvuI--20.0, 12.0, 8.2, 5.8; HindIII--23.0, 9.2, 3.9, 0.90, 0.65.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--3.9, 4.8, 5.5; BamHI--7.2, 10.

Cloned from DNA isolated from individual II-4 of the TOU family.

A 3.9 kb HindIII fragment from the 5' end of this insert, pH3.9EZZ, has been deposited independently as ATCC 61230, 61231. The 5' HindIII site is vector-derived.

A 2.8 kb BamHI fragment contains the exons encoding the epsilon constant region.

References

Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231

B224675：lambdaepsilon10EZZ

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