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YIplac211

規格:

價格:

編號:B218600

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YIplac211

商品貨號

B218600

Designations

YIplac211

Depositors

RD Gietz

培養基

Biosafety Level

Vector Information

Size (kb): 3.797

Vector: YIplac211 (plasmid)

Promoters: Promoter lac

Construction: pUC19

Marker(s):URA3,ampR

Construct size (kb): 3.797

Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI

Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII

Other unique sites: EcoRV AatII SspI NarI NsiI StuI ApaI NcoI

Applications

YI-type (integrating) shuttle vector

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

Comments

Restriction digests of the clone give the following sizes (kb): BglI--2.2, 1.6; PvuI--2.1, 1.8; PstI--3.9

One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).

The PstI restriction site from the URA3 gene of S. cerevisiae was removed by using oligo-directed mutagenesis.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106