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07-03 [pPM101]
07-03 [pPM101]
規格:
價格:
編號:B218610
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
07-03 [pPM101]
商品貨號
B218610
Designations
07-03 [pPM101]
Species
Homo sapiens, human
培養基
Applications
X linked segregation observed in 3 families with 31 progeny.
A 2.3 kb EcoRI fragment has been used to detect a BanII RFLP.
Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, BstEII, BstNI, CfoI, EcoRI, EcoRV, HaeIII, HindII, HindIII, HinfI, HpaII, KpnI, MspI, PstI, PvuII, RsaI, SacI, ScaI, TaqI, XbaI, XhoI, XmnI (tested against 10-12 unrelated Caucasians).
Vector
Construct size (kb): 50.0
Insert
DNA: genomic
Insert lengths(kb): 18.0
Gene product: DNA Segment, single copy [DXS79]
Alleles: A1, A2
Insert Size (kb)
18.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--23, 9.4 (arms); 18 (insert); EcoRI--20 (arm), 13 (arm/insert), 3.2, 3.0, 2.4, 1.8/1.75 doublet.
X linked segregation observed in 3 families with 31 progeny. Informative in CEPH pedigrees 21, 23, 1331, 1345, and IMR pedigree 884.
A 2.3 kb EcoRI fragment has been used to detect a BanII RFLP.
The 2.4 (2.3) kb EcoRI fragment is unique and detects the RFLP; other fragments contain repeats.
Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, BstEII, BstNI, CfoI, EcoRI, EcoRV, HaeIII, HindII, HindIII, HinfI, HpaII, KpnI, MspI, PstI, PvuII, RsaI, SacI, ScaI, TaqI, XbaI, XhoI, XmnI (tested against 10-12 unrelated Caucasians).
References

Murphy PD, et al. An anonymous single copy X-chromosome clone, DXS79, from Xq26-Xq28, identifies a moderately frequent RFLP. Nucleic Acids Res. 13: 3015, 1985. PubMed: 4000970

Murphy PD, Ruddle FH. Isolation and regional mapping of random X sequences from distal human X chromosome. Somatic Cell Mol. Genet. 11: 433-444, 1985. PubMed: 2994237

Patricia D Murphy, personal communication

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