黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pKT254omega-Ap [JF311] B218681
pKT254omega-Ap [JF311]
pKT254omega-Ap [JF311]
規(guī)格:
價格:
編號:B218681
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pKT254omega-Ap [JF311]
商品貨號
B218681
Designations
pKT254omega-Ap [JF311]
Depositors
J Frey
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 8.8999996185302730
Vector: pKT254omega-Ap (plasmid)
Construction: pHP45:omega, pKT254, pKT235 bla
Marker(s):ampR,kanR
Construct size (kb): 8.899999618530273
Features: marker(s): ampR, kanR
replicon: ColD of KT254
terminator: T4D gene 32
Applications
integrating vector
shuttle vector
vector permitting positive selection for integration
vector useful for interposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 2.7; HindIII--5.4, 2.6;
Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate.
One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria.
An EcoRI fragment containing omega was ligated to EcoRI-digested pKT254. The spcR gene was deleted (SphI/BssHII double digest) and replaced with a 1.75 kb BamHI/BalI fragment of pKT235 containing the ampicillin resistance gene.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679

Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930

Shipped
freeze-dried
  • 王經(jīng)理
    手機(jī)號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號：13626845108
    QQ：972239479