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ptkLUC+

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價格:

編號:B218901

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

ptkLUC+

商品貨號

B218901

Designations

ptkLUC+

Depositors

J Duschl, J Altschmied

培養基

Biosafety Level

Host

Distribution host: Escherichia coli HB101 (ATCC 33694)

Vector Information

Size (kb): 4.7109999656677250

DESCRIPTION OF VECTOR:
Intact vector size: 4.711
Type of vector: plasmid
Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI
Polylinker sites: HindIII SalI BamHI [tk-promoter luc+ SV40 poly (A)] ClaI
SmaI KpnI SacI
Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI
XmnI SspI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: TK, 37-200
reporter group: luciferase (luc+), ->, 238-1890
terminator: SV40 late polyadenylation, 1922-2143
MCS: ClaI...SacI, 2146-2168
replicon: pMB1, 2587-2587
marker(s): ampR, <-, 3345-4205
terminator: SV40 large T-antigen polyadenylation, 4337-4708

Vector: ptkLUC+ (plasmid)

Promoters: Promoter TK

Marker(s):ampR

Construct size (kb): 4.710999965667725

Features: marker(s): ampR
promoter: TK
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...SacI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation

Applications

reporter construct

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7.

The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.

The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.

The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.

The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214