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pRD112 plasmid in E. coli

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編號:B219006

品牌:Mingzhoubio

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產品名稱

pRD112 plasmid in E. coli

商品貨號

B219006

Designations

pRD112 plasmid in E. coli

Species

Aspergillus niger var. macrosporus Koaze, anamorph

培養基

Depositors

OmniGene, Inc., RR Yocum, OmniGene, Inc.

Applications

produces protein glucoamylase

Vector

Construct size (kb): 8.30

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGM30
Intact vector size: 8.800
Type of vector: plasmid
Vector end: XhoI Modification: BssHII adaptor
Vector end: XbaI
Cloning sites: XbaI XhoI
Polylinker sites:
Construction: pRY271, pGM11
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3
promoter: TPI
leader peptide: DEX4
coding sequence: DEX4
transcription terminator: TPI
replicon: pMB1
marker(s): ampR
replicon: 2 micron
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Aspergillus niger var. macrosporus
Gene name: glucoamylase
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert 5' end: BssHII
Insert 3' end: XbaI
Insert size (kb): 2.2
Cross references:

Insert lengths(kb): 2.20

Gene product: glucoamylase

Target Gene: glucoamylase

Insert Size (kb)

2.200

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): XbaI/XhoI--6.6, 2.1; SmaI--8.6; XbaI--8.6.

When transformed into yeast strain RD61-1D, pRD112 yields approximately 100 units of glucoamylase activity.

References

Maine GT, et al. Expression and secretion vector for yeast containing a glucoamylase signal sequence. US Patent 5,024,941 dated Jun 18 1991

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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