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pG53/T1
pG53/T1
規格:
價格:
編號:B219112
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pG53/T1
商品貨號
B219112
Designations
pG53/T1
GenBank Number

X55731

培養基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
A Tzagoloff
Applications
in another host, produces protein mitochondrial membrane protein, required for assembly of cytochrome oxidase
Vector
Construct size (kb): 20.29998970031738
Insert
DNA: genomic
Insert lengths(kb): 9.5
Gene product: mitochondrial membrane protein, required for assembly of cytochrome oxidase [COX11]
Target Gene: mitochondrial membrane protein, required for assembly of cytochrome oxidase
Insert Size (kb)
9.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BglII--6.6, 4.4 (doublet), 3.0, 2.0; EcoRI--5.2, 4.6 (doublet), 2.3 (doublet), 1.2; PstI--7.6, 5.8, 4.2, 1.7, 1.0;
HindIII--11.0, 4.4, 3.6, 1.2.
The insert contains the following restriction sites (approximate kb from the end distal to the vector HindIII site [nt of the published sequence]): HindIII--0.4, 4.8, 8.6; EcoRI--0.4 [42], 1.9 [1190], 7.4; BglII--1.5 [797], 3.6, 7.8.
The gene product is homologous to ORF3 of Paracoccus denitrificans.
The sequenced portion (the 1.87 kb BamHI/XbaI fragment) also contains an open reading frame transcribed in the opposite orientation.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Tzagoloff A, et al. Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3. EMBO J. 9: 2759-2764, 1990. PubMed: 2167832

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