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pVL-RabGGTbeta
pVL-RabGGTbeta
規格:
價格:
編號:B219165
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pVL-RabGGTbeta
商品貨號
B219165
Designations
pVL-RabGGTbeta
GenBank Number

L10416

培養基
Species
Rattus norvegicus, rat
Depositors
MC Seabra, S Armstrong
Applications
in suitable host, produces protein rab geranylgeranyl transferase beta Rab geranylgeranyl transferase, beta subunit
Vector
Construct size (kb): 11.0
Insert
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: brain
Gene product: rab geranylgeranyl transferase beta(Rab geranylgeranyl transferase, beta subunit) [GNGNT2B*]
Target Gene: rab geranylgeranyl transferase beta
Insert Size (kb)
1.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; BamHI--9.8, 0.9; XhoI--7.7, 2.3.
The insert contains the following restriction sites (approximate kb from the 5' end): XhoI--0.07; BglII--0.59,1.14; BamHI--0.87; BclI--1.29.
When pVL-RabGGTalpha and pVL-RabGGTbeta (ATCC 87154, 87155) are used to generate recombinant baculoviruses and infect Sf9 cells, the active dimer is produced.
References

Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464

Luckow VA, Summers MD. Signals Important for high-level expression of foreign genes in Autographa californica Nuclear Polyhedrosis Virus expression vectors. Virology 167: 56-71, 1988. PubMed: 3142147

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