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HFBDN96

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價格:

編號:B219748

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

HFBDN96

商品貨號

B219748

Designations

HFBDN96

GenBank Number

M74718

T06068

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SEF2A1*
Genomic copy number: unique
Gene name: transcription factor SEF2-1A
Contains complete coding sequence?: N
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M74718; EST: EST03957
Nucleotides 86-286 of the insert correspond to
nucleotides 1836-1636 of M74718 with 97% identity.
DNA Seq. Acc.: T06068; EST: EST03957; Primer: M13-21

Insert lengths(kb): 1.5

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: transcription factor SEF2-1A [SEF2A1*]

Insert Size (kb)

1.5

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sized (kb): EcoRI--3.1, 1.49.

Based on the alignment with GenBank M74718 (1840 nt, CDS <1-1488) and the insert size, this clone contains part of the coding region.

References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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