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pM220

規格:

價格:

編號:B219791

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pM220

商品貨號

B219791

Designations

pM220

Depositors

ZymoGenetics, Inc., JD Kelly, MJ Murray, ZymoGenetics, Inc.

培養基

U.S. Patent

4,766,073

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Host

Distribution host: Escherichia coli RR1; K-12 RR1

Vector Information

Size (kb): 8.1999998092651370

Vector: pM220 (plasmid)

Promoters: Promoter TPI

Construction: TPI promoter, MFalpha1, 2 micron

Marker(s):ampR

Construct size (kb): 8.199999809265137

Features: marker(s): ampR
promoter: TPI
replicon: pMB1, 2 micron

Applications

contains easily purifiable cassette(s) for construction

Comments

Contains EcoRI, PstI, HindIII, BamHI and BglII sites.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8, 1.3; HindIII--8.2; BamHI--8.2.

A plasmid containing the S. cerevisiae triosephosphate isomerase promoter fused to the signal sequence for mating factor alpha 1 on a BglII/HindIII fragment.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Murray MJ, Kelly JD. Expression of biologically active PDGF analogs in eucaryotic cells. US Patent 4,766,073 dated Aug 23 1988

Shipped

frozen

Shipping Information

Distributed: freeze-dried

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