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pYe(CEN3)30
pYe(CEN3)30
規格:
價格:
編號:B219805
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pYe(CEN3)30
商品貨號
B219805
Designations
pYe(CEN3)30
Depositors
Amgen, Inc., BJ Ratzkin, Amgen, Inc.
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli JA228
Vector Information
Size (kb): 6.3000001907348630
Vector: pYe(CEN3)30 (plasmid)
Construction: YRp7'
Marker(s):TRP1,ampR
Construct size (kb): 6.300000190734863
Features: marker(s): ampR, TRP1
replicon: pMB1, ARS1
centromere: CEN3
Applications
YC-type (centromeric) shuttle vector
shuttle vector
Comments
Contains unique BamHI, BglII, XbaI, NruI, and SalI sites, with XbaI and BglII inactivating TRP1.
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglII--6.3; SalI--6.3; EcoRI--4.6, 1.7.
Demonstrates mitotic stability increased over YRp7'; 60-90% of transformed S. cerevisiae J17 maintain the Trp+ phenotype after 20 generations.
Constructed by inserting a 627 bp BamHI/Sau3AI fragment containing CEN3 into the BamHI site of YRp7'.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Patel AC, Ratzkin BJ. Materials and methods for controlling plasmid copy number and stability. US Patent 4,935,350 dated Jun 19 1990

Fitzgerald-Hayes M, et al. Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs. Cell 29: 235-244, 1982. PubMed: 7049398

Barry J Ratzkin, personal communication

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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