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pC21/3c
pC21/3c
規格:
價格:
編號:B219912
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pC21/3c
商品貨號
B219912
Designations
pC21/3c
GenBank Number

M13936

培養基
Species
Homo sapiens, human
Depositors
PC White
Applications
Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: fetal adrenal gland
Gene product: cytochrome P450, subfamily XXI (steroid 21-hydroxylase, congenital adrenal hyperplasia) [CYP21]
Alleles: A2, B2, A2, B2, A1, B1, A1, B1, C2, C2, C1, C1
Insert Size (kb)
2.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.2; XhoI--3.0, 2.3; PstI--1.95, 1.2, 0.53, 0.47 + others; HindIII--5.2; ClaI--5.2.
This encodes the complete cDNA except for about 30 bp at the 5' end. The poly(A) signal is 21 bp upstream of the poly(A) sequence (42 bp).
Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications. Detects bands of 10 kb (A) and 12 kb (B) in BglII digests.
References

White PC, et al. Two genes encoding steroid 21-hydroxylase are located near the genes encoding the fourth component of complement in man. Proc. Natl. Acad. Sci. USA 82: 1089-1093, 1985. PubMed: 2983330

White PC, et al. Structure of human steroid 21-hydroxylase genes. Proc. Natl. Acad. Sci. USA 83: 5111-5115, 1986. PubMed: 3487786

Werkmeister JW, et al. Frequent deletion and duplication of the steroid 21-hydroxylase genes. Am. J. Hum. Genet. 39: 461-469, 1986. PubMed: 3490178

Amor M, et al. Mutation in the CYP21B gene (Ile-172-->Asn) causes steroid 21-hydroxylase deficiency. Proc. Natl. Acad. Sci. USA 85: 1600-1604, 1988. PubMed: 3257825

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