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lambdaMGU2 <em>C. elegans</em> cDNA library
lambdaMGU2 <em>C. elegans</em> cDNA library
規格:
貨期:
編號:B219940
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdaMGU2 C. elegans cDNA library
商品貨號
B219940
Designations
lambdaMGU2 C. elegans cDNA library
Species
Caenorhabditis elegans Maupas
Depositors
IN Maruyama
Applications
To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046[pCRE1], ATCC 77368) and select for ampicillin resistance.
Strain of C. elegans used for constructing this library is C. elegans N2 which is a wild type strain.
Vector
Library information:
Genome: Caenorhabditis elegans
Strain: N2
Type of insert: cDNA
Vector: lambdaMGU2
Insert size range (kb): 2-3 kb average
Vector ends: Modification: BamHI
Number of independent recombinants: 1.0 x 107
Titer: 5 x 107 pfu/mL

Vector information:
Vector: lambdaMGU2
Vector type: phage
Intact vector size (kb): 41.7
Construction: lambda2690, pMGU
Features:
Insert detection: Spi+
Marker: ampR
Replicon: lambda, pMB1, m13
Related Products
Component of: ATCC 77370
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Frozen bacteria-free phage lysate
Comments
Strain of C. elegans used for constructing this library is C. elegans N2 which is a wild type strain.
Single-stranded DNA may be recovered from phagemid constructs using M13KO7 helper phages.
To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046[pCRE1], ATCC 77368) and select for ampicillin resistance. The pMGU product is 4.185 kb.
Inserts can be amplified using the following primers flanking the BamHI cloning site: upstream 5'-AAGAGGCAGAACTGGCAG-3' and downstream 5'-ATCGATGCATAGCGATTC-3'.
The order of the major features in the cloning region of the lambda vector is: lambda J - SmaI - SalI - loxP - EcoRI - M13 ori - ampR - pMB1 ori - HindIII - 3'gam/BamHI/5'gam - XhoI - loxP - SalI - lambda N.
Efficiency of phagemid recovery is approximately 20%. Plasmid pCRE1 may be a low level contaminant, but is easily distinguished from pMGU DNA.
References

Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972

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