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pI304

規格:

價格:

編號:B219941

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pI304

商品貨號

B219941

Designations

pI304

Depositors

KW Minton, HD Braymer

培養基

Biosafety Level

Vector Information

Size (kb): 14.5000000000000000

Vector: pI304 (plasmid)

Construction: pUE10, pKK232-8

Marker(s):ampR

Construct size (kb): 14.5

Features: insert detection: CAT
marker(s): ampR
replicon: pMB1, pUE10

Applications

promoter-cloning vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--14.5; SalI--14.5; HindIII--9.4, 5.1.

Promoter-cloning vector for Deinococcus radiodurans. Inserts with promoter function permit expression of chloramphenicol resistance.

The order of the major features in this plasmid is: pUE10 replicon - cmlR - pMB1 ori - ampR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940

Shipped

freeze-dried

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