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YEpFAT10

規格:

價格:

編號:B219958

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YEpFAT10

商品貨號

B219958

Designations

YEpFAT10

Depositors

DJ Stillman, K Runge

培養基

Biosafety Level

Vector Information

Size (kb): 6.7709999084472660

Vector: YEpFAT10 (plasmid)

Promoters: Promoter for in vitro transcription T7

Construction: pVZ1

Marker(s):TRP1,ampR,LEU2-d

Construct size (kb): 6.770999908447266

Features: marker(s): LEU2-d
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron ori/LEU2-d
replicon: pMB1
MCS: ApaI...SacII
MCS: NruI...SphI

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI-- 4.2, 2.9; BamHI-- 7.0; SalI-- 7.0.

Selection for TRP1 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.

Similarly, the NruI, BamHI, SalI and SphI are adjacent and can be used for directional cloning. The vector was constructed by Kurt Runge.

One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Conrad MN, et al. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63: 739-750, 1990. PubMed: 2225074

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped

freeze-dried

Shipping Information

?Freeze dried stock of Escherichia coli containing the plasmid.

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