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pVH-3
pVH-3
規(guī)格:
價(jià)格:
編號(hào):B220000
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pVH-3
商品貨號(hào)
B220000
Designations
pVH-3
Species
Potato spindle tuber viroid
培養(yǎng)基
Depositors
RA Owens
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 0.3400000035762787
Insert Size (kb)
0.340
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains internal sites for AvaII, AluI, BamHI and SmaI.
Restriction digests of the clone give the following sizes (kb): HaeIII--0.64, 0.54, 0.48, 0.38, plus smaller fragments; EcoRI--4.7; PstI--4.7; EcoRI/PstI--4.0, 0.74; EcoRI/PvuII--2.5, 2.4.
Noninfectious unless released from plasmid by HaeIII digestion (and even then probably noninfectious).
Full-length PSTV cDNA with HaeIII ends was cloned into the HindIII site of pBR322 using HindIII linkers.
Classification
Pospiviroid
References

Cress DE, et al. Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Res. 11: 6821-6835, 1983. PubMed: 6314259

Robert A Owens, personal communication

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