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MIA PaCa-2
MIA PaCa-2
規格:
價格:
編號:B220097
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MIA PaCa-2
商品貨號
B220097
Organism
Homo sapiens, human
Tissue
pancreas
培養基
Cell Type
epithelial cell (KRAS CRM)
Product Format
frozen
Morphology
attached epithelial with floating rounded cells
Culture Properties
adherent, single cells and loosely attached clusters
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma
Age
65 years
Gender
Male
Ethnicity
Caucasian
Applications
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. 
Intended Use

Certified Reference Material produced under an ISO 17034 accredited process.


    ACLASS Accredited Reference Material Producer AR-1384

Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
Derivation
The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).
Clinical Data
65 years
Caucasian
male
Genes Expressed
human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Cellular Products
human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Comments
Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

Item has been tested for KRAS mutation (p.G12C c.34G>T)

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.

The line is sensitive to asparaginase.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15
Isoenzymes
G6PD, B
Name of Depositor
A Yunis
Deposited As
Homo sapiens
Year of Origin
1975
References

Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918

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