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pRS421

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價格:

編號:B220100

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRS421

商品貨號

B220100

Designations

pRS421

Depositors

JD Boeke, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine

培養基

Biosafety Level

Host

Escherichia coli HB101 (ATCC 33694)

Vector Information

Size of construct (kb): 6.233
Markers: ampR, MET15
DNA Seq. Accession number: U93716
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI XhoI ApaI KpnI
Construction: pRS401, YEp24
Host range: S. cerevisiae; E. coli

Features (with position when available):
marker(s): MET15, 421-1270
replicon: f1, 1860-2316
promoter for in vitro transcription: T7, 2486-2505
MCS: KpnI...SacI, 2513-2615
promoter for in vitro transcription: T3, 2632-2651
insert detection: lacZ', 2317-2676
promoter: lac, 2721-2749
replicon: pMB1, 3075-3075
marker(s): ampR, 3833-4693
replicon: 2 micron, 4825-6166

Applications

YE-type (episomal) shuttle vector

Vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0.

met15 phenotype produces brown colonies when grown on Pb containing media.

MET15, MET17 and MET25 are synonymous.

This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

References

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801