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HFBCK22
HFBCK22
規(guī)格:
價(jià)格:
編號(hào):B220219
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HFBCK22
商品貨號(hào)
B220219
Designations
HFBCK22
GenBank Number

M85571

培養(yǎng)基
Species
Homo sapiens, human
Applications
The predicted product size is (bp): 149. The observed product size(s) is(are) (bp): 156 (human).
Vector
Construct size (kb): 4.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCI
Gene name: protein kinase C, iota
Contains complete coding sequence?: N
Chromosome: X; Localization: X q21.3
Genome: Homo sapiens
Gene symbol: DXS1179E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: N
Chromosome: X; Localization: X
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: L33881; EST: EST02087
Nucleotides 1-397 of the insert correspond to
nucleotides 1293-897 of L33881 with 98% identity.
DNA Seq. Acc.: M85571; EST: EST02087; Primer: M13 Forward;
THC id: THC82066
Insert lengths(kb): 1.100000023841858
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: protein kinase C, iota [PRKCI]
Insert Size (kb)
1.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.1; BamHI--4.0; HindIII--3.3, 0.4, 0.35; PstI--2.9, 1.1; PvuII--2.45, 1.55.
EST02087 can be detected in genomic DNA using the following oligonucleotides (5'-3'): TGCTTCTCTGTCTGTACCCA and GATTTGCTCCGGGTAATAGG.
Based on the size of the insert and the alignment with GenBank L33881 (2261 nt, CDS 205-1968), the clone appears to contain part of the coding region and some of the 3' untranslated region.
The predicted product size is (bp): 149. The observed product size(s) is(are) (bp): 156 (human).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

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