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pBT7
pBT7
規(guī)格:
價格:
編號:B220260
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBT7
商品貨號
B220260
Designations
pBT7
Depositors
IN Maruyama
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 2.7539999485015870
Vector: pBT7 (plasmid)
Promoters: Promoter lac
Construction: pUC18, bacteriophage T7
Marker(s):ampR
Construct size (kb): 2.753999948501587
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac, T7 gene 10'
replicon: pMB1
Applications
expression vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): NdeI--2.75; HindIII--2.75; EcoRI--2.75.
Expression vector based on a bacteriophage T7 RNA polymerase/promoter system and allowing visual detection of inserts using lacZalpha complementation.
The T7 promoter sequence has a T->A mutation at the 5' end that does not appear to affect expression.
The order and orientations of the major features of the plasmid are: <- lacZ' - HindIII - T7 promoter -> - NdeI/MCS/EcoRI - <-pMB1 ori - <- ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Maruyama IN, et al. A bacteriophage T7-based expression vector, pBT7, with color selection for the recombinant. Gene 131: 79-82, 1993. PubMed: 8370543

Shipped
freeze-dried
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