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pAGRBglR2 [NEB731]
pAGRBglR2 [NEB731]
規格:
價格:
編號:B220459
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAGRBglR2 [NEB731]
商品貨號
B220459
Designations
pAGRBglR2 [NEB731]
GenBank Number

I13180

培養基
Species
Bacillus globigii Migula
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
produces protein modification methylase BglII
produces protein restriction endonuclease BglII
Vector
Construct size (kb): 8.5
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: restriction endonuclease BglII [hsdM]
Target Gene: modification methylase BglII, restriction endonuclease BglII
Insert Size (kb)
1.800
Media
ATCC Medium 1065 (see below) plus ampicillin (100.0 ug/ml) plus kanamycin (50.0 ug/ml) plus tetracycline (10.0 ug/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of pSYXBglM (weaker) give the following sizes (kb): EcoRI--uncut; BamHI--7.2, 1.6; HindIII--4.8, 3.7; PstI--8.2.
Restriction digests of pAGRBglR2 give the following sizes (kb): EcoRI--uncut; BamHI--7.2; HindIII--7.4; PstI--7.8.
This contains two plasmids, one encoding the methylase and one encoding the restriction endonuclease.
To optimize growth and expression, grow in LB containing carbenicillin, kanamycin, and tetracycline at 30C until late log phase. Induce with IPTG and grow 20 more hrs at 30C until harvest.
References

Brooks JE, et al. Method for cloning and producing the BglII restriction endonuclease and modification methylase. US Patent 5,434,068 dated Jul 18 1995

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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