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pEGsacIIRMC5 [NEB673]
pEGsacIIRMC5 [NEB673]
規格:
價格:
編號:B220467
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pEGsacIIRMC5 [NEB673]
商品貨號
B220467
Designations
pEGsacIIRMC5 [NEB673]
Species
Streptomyces achromogenes Okami and Umezawa
培養基
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
produces protein modification methylase SacII
produces protein restriction endonuclease SacII
Insert
DNA: genomic
Insert lengths(kb): 3.650000095367432
Gene product: restriction endonuclease SacII [hsdM]
Target Gene: modification methylase SacII, restriction endonuclease SacII
Insert Size (kb)
3.650
Media
ATCC® Medium 1339: R2 YE medium
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried S. lividans containing the plasmid

Comments
The insert contains the following restriction sites (approximate kb from the 5' end): ApaI--0.6, 2.1; BamHI--0.9, 3.0; BglII--2.2; NcoI--1.1, 1.8, 2.8; PstI--2.5.
The methylase gene is contained in the 2.5 kb HindIII/PstI fragment.
Verified to be viable in R2 YE medium + 50 ug/ml thiostrepton.
References

Guthrie EP, Meda MM. Method for producing and cloning SacII restriction endonuclease and methylase. US Patent 5,288,696 dated Feb 22 1994

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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