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pK194

規(guī)格:

貨期:

編號(hào):B220498

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

產(chǎn)品名稱	pK194
商品貨號(hào)	B220498
Designations	pK194
Depositors	RK Holmes
Biosafety Level	1
Vector Information	Size (kb): 2.4319999217987060 Vector: pK194 (plasmid) Promoters: Promoter lac Construction: pK184 M13mp19 Marker(s):kanR Construct size (kb): 2.431999921798706 Features: insert detection: lacZ' marker(s): kanR promoter: lac replicon: p15A terminator: none
Applications	A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication.
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; HindIII--2.4; PstI--2.4; BamHI--2.4; XbaI--2.4. A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication. Constructed by M.G. Jobling. pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence.
Media	ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions	Temperature: 37.0°C
References	Jobling MG, Holmes RK. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZalpha and pUC18 or pUC19 multiple cloning sites. Nucleic Acids Res. 18: 5315-5316, 1990. PubMed: 2402474 Randall K Holmes, personal communication
Shipped	freeze-dried

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