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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pK194
pK194
規格:
價格:
編號:B220498
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pK194
商品貨號
B220498
Designations
pK194
Depositors
RK Holmes
培養基
Biosafety Level
1
Vector Information
Size (kb): 2.4319999217987060
Vector: pK194 (plasmid)
Promoters: Promoter lac
Construction: pK184 M13mp19
Marker(s):kanR
Construct size (kb): 2.431999921798706
Features: insert detection: lacZ'
marker(s): kanR
promoter: lac
replicon: p15A
terminator: none
Applications
A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; HindIII--2.4; PstI--2.4; BamHI--2.4; XbaI--2.4.
A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication.
Constructed by M.G. Jobling.
pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Jobling MG, Holmes RK. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZalpha and pUC18 or pUC19 multiple cloning sites. Nucleic Acids Res. 18: 5315-5316, 1990. PubMed: 2402474

Randall K Holmes, personal communication

Shipped
freeze-dried
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