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pKLy6.1-2R

規(guī)格:

價格:

編號:B220679

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pKLy6.1-2R

商品貨號

B220679

Designations

pKLy6.1-2R

GenBank Number

X04653

培養(yǎng)基

Species

Mus musculus, mouse

Depositors

AL Bothwell

Vector

Construct size (kb): 3.5

Insert

DNA: cDNA

Insert lengths(kb): 0.7609999775886536

Tissue: MethA fibrosarcoma cell line

Gene product: lymphocyte antigen-6 [DAG]

Alleles: 6.1, 6.1, 6.1, 6.2, 6.2, 6.2, 6.1, 6.1, 6.2, 6.2, 6.1, 6.2

Insert Size (kb)

0.761

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BglI--2.3, 1.15; EcoRI--2.7, 0.8; PstI--3.2, 0.35; PvuII--2.4, 1.15; SacI--2.9, 0.3 (doublet).

The insert contains the following restriction sites (nt from the 5' end): SacI--160, 467; PstI--480.

Encodes Ly-6E.1 (Ly-6a allele) recognized by the SK70.94 and HD42 monoclonal antibodies.

Contains the complete coding sequence (nt 89-492) and extends from a linker-derived EcoRI site at the 5' end to a naturally occurring EcoRI site in the 3' untranslated region.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

LeClair KP, et al. Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J. 5: 3227-3234, 1986. PubMed: 3028776

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