Comments
The suggested host for vector amplification is E. coli C600 (ATCC 23724) and for library amplification is E. coli JC8679 (ATCC 47001).
Vector for efficient transduction of mammalian cells with a cDNA library constructed with the pcD (Okayama and Berg) expression vector.
Cloning capacity: 12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector.
The order of the major features in this bacteriophage is: lambda762/deltab - HindIII - 0.5 kb SV40 fragment, map units 0.86-0.95 - reddelta - SalI - SV40 ori - SV40 early promoter - SV40 late 19S and 16S splice junctions - neo (Tn5) - SV40 late poly(A)
- pMB1 ori - ampR - lambda762/KH54/lambda ori/nin5/QSR.
