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pRY252
pRY252
規格:
價格:
編號:B220731
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRY252
商品貨號
B220731
Designations
pRY252
Depositors
RR Yocum
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli YMC9
Vector Information
Size (kb): 9.3000001907348630
Vector: pRY252 (plasmid)
Promoters: Promoter CYC1
Construction: pBR322
Marker(s):ampR G418R URA3
Construct size (kb): 9.300000190734863
Features: marker(s): G418R
marker(s): URA3
marker(s): ampR
promoter: CYC1
replicon: 2 micron
replicon: pMB1
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 2.3; SalI--9.3; PvuII--uncut; XbaI--9.3; XhoI--8.9, 0.4.
YE-type shuttle vector conferring G418 resistance under control of the CYC1 promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995

Shipping Information
Distributed: freeze-dried
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