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pUK21-NotI

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貨期:

編號:B220830

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產(chǎn)品名稱	pUK21-NotI
商品貨號	B220830
Designations	pUK21-NotI
Depositors	DJ Stillman
Biosafety Level	1
Host	Escherichia coli HB101
Vector Information	Intact vector size: 3.250 Type of vector: plasmid Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeI SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI Construction: pUC21 and pFA6b Features (with orientation and position when available): Vector: pUK21-NotI (plasmid) Construction: pUC21 and pFA6b Marker(s): kanR Construct size (kb): 3.25
Applications	Cloning vector
Comments	Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25. This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b.
Media	ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions	Temperature: 37°C
References	Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257 Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518 David J Stillman, personal communication
Shipped	freeze-dried

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