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pUK21-NotI

規格:

價格:

編號:B220830

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUK21-NotI

商品貨號

B220830

Designations

pUK21-NotI

Depositors

DJ Stillman

培養基

Biosafety Level

Host

Escherichia coli HB101

Vector Information

Intact vector size: 3.250

Type of vector: plasmid
Cloning sites: StuI XhoI HindIII BsiWI PstI SalI PmeI SacI EcoRI SfiI XbaI
Polylinker sites: SpeI StuI XhoI EcoRV NotI PvuII HindIII BsiWI PstI SalI PmeI SacI EcoRV SpeI SfiI NotI XbaI SpeI ClaI
Construction: pUC21 and pFA6b

Features (with orientation and position when available):

Vector: pUK21-NotI (plasmid)

Construction: pUC21 and pFA6b

Marker(s): kanR

Construct size (kb): 3.25

Applications

Cloning vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRV--3.25; XbaI--3.25; PvuI--2.0, 1.25.

This plasmid is derived from pUC21 by deletion of the BamHI-BglII fragment and insertion of the NotI polylinker fragment from pFA6b.

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Growth Conditions

Temperature: 37°C

References

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

David J Stillman, personal communication

Shipped

freeze-dried

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