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p519gfp
p519gfp
規格:
價格:
編號:B220963
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p519gfp
商品貨號
B220963
Designations
p519gfp
Depositors
AE Goodman, AG Matthysse
培養基
Biosafety Level
1
Vector Information
Size (kb): 9.0000000000000000
Vector: p519gfp (plasmid)
Promoters: Promoter for expression lac
Marker(s):kanR
Features: marker(s): kanR, neoR
other: mob
promoter for expression: lac
MCS: HindIII/.../XbaI
restriction site: EcoRI
restriction site: NdeI
ribosome-binding site: GFP
coding sequence: GFPmut2
Applications
contains easily purifiable cassette(s) for construction green fluorescent protein (mutant)
vector permitting visual detection of activity in colonies green fluorescent protein (mutant)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--9.0; EcoRI--9.2; EcoRI/HindIII--6.7, 1.7, 0.7; PstI--5.6, 1.8, 1.2.
The promoterless reporter GFPmut2 cassette, with its ribosome binding site, can be recovered as a ndIII/EcoRI (5'/3') fragment (approximately 740 bp).
A broad host range mob+ plasmid with GFPmut2 expression controlled by a lac promoter.
Cloning into the SphI, PstI, AccI, HincII, SalI, XbaI, or NdeI sites will probably affect GFPmut2* expression as it would separate the CDS or RBS from the lac promoter. This has not yet been tested.
Media
ATCC® Medium 1634: LB medium (ATCC medium 1065) with 50 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Matthysse AG, et al. Construction of GFP vectors for use in gram negative bacteria other than E. coli. FEMS Microbiol. Lett. 145: 87-94, 1996. PubMed: 8931331

Keen NT, et al. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197, 1988. PubMed: 2853689

Cormack BP, et al. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38, 1996. PubMed: 8707053

Shipped
freeze-dried
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