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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pTKbeta
pTKbeta
規格:
價格:
編號:B221014
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pTKbeta
商品貨號
B221014
Designations
pTKbeta
Depositors
GR Macgregor
培養基
Biosafety Level
1
Host
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 7.5999999046325680
DESCRIPTION OF VECTOR:
Intact vector size: 7.600
Type of vector: plasmid
Cloning sites: XbaI NotI SalI SphI HindIII
Polylinker sites: EcoRI BamHI XbaI SalI PstI SphI HindIII
Construction: pUC19, pC4AUG beta-gal, pL1, pXT1
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: SV40 late
Vector: pTKbeta (plasmid)
Promoters: Promoter HSV TK
Construction: pUC19, pC4AUG beta-gal, pL1, pXT1
Marker(s):ampR
Construct size (kb): 7.599999904632568
Features: insert detection: lacZ'
marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: SV40 late
Applications
contains easily purifiable cassette(s) for construction
reporter construct
vector for other uses
vector permitting visual detection of activity in colonies
Comments
Restriction digest of the clone give the following sizes (kb): EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5, 2.7, 0.9; NotI--4.4, 3.2; BamHI--3.8 (doublet).
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
Designed to express low levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
The order of the major features in this plasmid is: pUC19 - EcoRI - HSV thymidine kinase promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19.
Media
ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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