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HFBCX08
HFBCX08
規格:
價格:
編號:B221061
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HFBCX08
商品貨號
B221061
Designations
HFBCX08
GenBank Number

M85990

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RAB5B
Genomic copy number: unique
Gene name: RAB5B, member RAS oncogene family
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 q13
Genome: Homo sapiens
Gene symbol: RAB5B
Genomic copy number: unique
Gene name: guanine nucleotide-binding protein Rab5B, ras-oncogene
related
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 q13
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M85990; EST: EST02515; Primer: M13 Forward;
THC id: THC209023
DNA Seq. Acc.: T05431; EST: EST03320; Primer: M13 Reverse;
THC id: THC209023
DNA Seq. Acc.: X54871; EST: EST02515; THC id: THC209023
Nucleotides 6-291 of the insert correspond to
nucleotides 1-284 of X54871 with 99% identity.
Insert lengths(kb): 2.400000095367432
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: RAB5B, member RAS oncogene family( guanine nucleotide-binding protein Rab5B, ras-oncogene related, GTP-binding protein rab5B) [RAB5B]
Insert Size (kb)
2.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 2.4.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.4; BamHI--5.4; PvuII--2.6, 1.7, 0.7, 0.5.
References

Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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