精品丰满少妇一区二区三区,午夜av在线,精品久久久久久

pCM56

規格:

價格:

編號:B221251

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pCM56

商品貨號

B221251

Designations

pCM56

GenBank Number

X02981

培養基

Species

Escherichia coli bacteriophage T3

Depositors

Univ. Med. & Dent. New Jersey, LN Stephenson, Univ. Med. & Dent. New Jersey

Applications

produces protein RNA polymerase

Vector

Construct size (kb): 8.48

Name of vector: pCM53
Intact vector size: 5.758
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: pAR1234 (deletion of the 346 bp HindIII/BamHI fragment
following the lacUV5 promoter)
Host range: Escherichia coli

Features (with orientation and position when available):
restriction site: BamHI, 3-3
replicon: pMB1, ← 2128-2128
marker(s): ampR, ← 2923-3783
repressor gene: lacI, → 4106-5180
promoter for expression: lacUV5, → 5273-5273

Insert

Genome: bacteriophage T3
Gene symbol: gene 1
Gene name: RNA polymerase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: Modification: BamHI linker
Insert end: Modification: BamHI linker
Insert size (kb): 2.712
Cross references: DNA Seq. Acc.: X02981
Nucleotides 1-2712 of the insert correspond to
nucleotides 96-2807 of X02981.

Insert lengths(kb): 2.71

Gene product: RNA polymerase [gene 1]

Target Gene: RNA polymerase

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Frozen glycerol stock of E. coli containing the plasmid.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.55, 2.7; HindIII--9.4; NdeI--6.55, 2.7.

Insert contains the following restriction sites (approximate kb from the 5' end): HincII--0.3, 1.0, 1.7; NdeI--1.9; StuI--0.4.

Cloned from a 5.9 kb MboI fragment of bacteriophage T3. The fragment was digested with MstI and AflII and treated with Bal31 to remove promoter sequences. Expression in this construct is regulated by the lacUV5 promoter and inducible by IPTG.

References

McAllister WT. Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids. US Patent 5,037,745 dated Aug 6 1991

Morris CE, et al. Cloning and expression of the bacteriophage T3 RNA polymerase gene. Gene 41: 193-200, 1986. PubMed: 3011596

McGraw NJ, et al. Sequence and analysis of the gene for bacteriophage T3 RNA polymerase. Nucleic Acids Res. 13: 6753-6766, 1985. PubMed: 3903658

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479