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pRRS-SacIR+ [NEB 963]
pRRS-SacIR+ [NEB 963]
規格:
價格:
編號:B221287
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRRS-SacIR+ [NEB 963]
商品貨號
B221287
Designations
pRRS-SacIR+ [NEB 963]
Species
Streptomyces achromogenes Okami and Umezawa
培養基
Depositors
New England Biolabs, Inc., GD Williams, New England Biolabs, Inc.
Applications
This is an E. coli containing 3 plasmids - pRRS-SacIR+, pLG-SacIM+ and pLG339- that produces the restriction endonuclease and modification methylase SacI.
Insert
DNA: genomic
Gene product: restriction endonuclease SacI
Gene product: modification methylase SacI
Media
ATCC Medium 1065 (see below) plus ampicillin (100.0 ug/ml) plus kanamycin (50.0 ug/ml) plus tetracycline (10.0 ug/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmids

Comments
This is an E. coli containing 3 plasmids - pRRS-SacIR+, pLG-SacIM+ and pLG339- that produces the restriction endonuclease and modification methylase SacI.
This should be grown on LB media containing ampicillin (50 ug/ml), kanamycin (50 ug/ml) and tetracycline (15 ug/ml) to maintain all plasmids.
This culture must be grown at temperatures no greater than 30C to maintain stability.
References

Xu SY, Xiao J. Method for cloning and producing the SacI restriction endonuclease. US Patent 5,532,153 dated Jul 2 1996

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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