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pCLL34
pCLL34
規格:
價格:
編號:B221474
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCLL34
商品貨號
B221474
Designations
pCLL34
Depositors
American Cyanamid Co., JS Feitelson, American Cyanamid Co.
培養基
Isolation
Constructed by inserting a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker into the BamHI site of pARC1.
Applications
Promoter-cloning vector
Vector permitting visual detection of activity in colonies
Promoter-cloning vector permitting visual detection of activity by expression of a brown pigment.
Vector
Size (kb): 19.7999897003173800
Vector: pCLL34 (plasmid)
Construction: pARC1, pIJ486
Marker(s):tsrR
Construct size (kb): 19.79998970031738
Features: insert detection: brown
marker(s): tsrR
terminator: fd
Media
ATCC® Medium 1339: R2 YE medium
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--16.71, 3.41.
The additional 35 bp of MCS between the BamHI site and the start of the brown genes do not interfere with gene expression.
Contains plasmid pCLL34
Constructed by inserting a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker into the BamHI site of pARC1.
The order of the major features in this plasmid is: fd terminator - MCS - brown genes - tsrR.
References

Feitelson JS. Promoter-probe plasmid for analysis of transcriptional regulation. US Patent 4,994,389 dated Feb 19 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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