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pRat alpha 1
pRat alpha 1
規格:
價格:
編號:B221566
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRat alpha 1
商品貨號
B221566
Designations
pRat alpha 1
GenBank Number

M14511

培養基
Species
Rattus norvegicus, rat
Depositors
JB Lingrel
Applications
isoform-specific probe ATPase, Na+/K+ transporting, alpha 1 polypeptide
Vector
Construct size (kb): 3.118000030517578
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: ATP1A1
Gene name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Contains complete coding sequence?: N
Strain: CD
Tissue: brain
Type of DNA: cDNA
Insert 5' end: NarI
Insert 3' end: StuI
Insert size (kb): 0.332
Cross references: DNA Seq. Acc.: M14511
Nucleotides 1-332 of the insert correspond to
nucleotides 89-421 of M14511.
Insert lengths(kb): 0.3319999873638153
Tissue: brain
Gene product: ATPase, Na+/K+ transporting, alpha 1 polypeptide [ATP1A1]
Target Gene: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Insert Size (kb)
0.332
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7, 0.35; BglI--1.9, 1.2; PvuII--2.3, 0.3 (doublet).
The insert contains the following restriction sites (approximate kb from the 5' end): ApaI--0.13; NcoI--0.15; PvuII--0.19.
References

Young RM, Lingrel JB. Tissue distribution of mRNAs encoding the alpha isoforms and beta subunit of Rat Na+, K+-ATPase. Biochem. Biophys. Res. Commun. 145: 52-58, 1987. PubMed: 3036133

Orlowski J, Lingrel JB. Tissue-specific and developmental regulation of Rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs. J. Biol. Chem. 263: 10436-10442, 1988. PubMed: 2839491

Shull GE, et al. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha subunit from Rat brain. Biochemistry 25: 8125-8132, 1986. PubMed: 3028470

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