Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2 to 6 days
Zheng ZM, et al. First isolation of enterovirus type 71 from vesicle fluid of an adult patient with hand-foot-mouth disease in China. Virologica Sinica. 4: 375-382, 1989.
Zheng ZM, et al. Comparison of enterovirus 71 (E71) isolated from a patient with hand-foot-and-mouth disease in China to prototype E71 BrCr strain by polymerase chain reaction using a unique primer pair. Clin. Diagn. Virol. 1: 137-139, 1993. PubMed: 15566727
Zheng ZM, et al. Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5'-noncoding region. J. Med. Virol. 47: 161-167, 1995. PubMed: 8830120
