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HFBCI36
HFBCI36
規格:
價格:
編號:B221768
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HFBCI36
商品貨號
B221768
Designations
HFBCI36
GenBank Number

M85450

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: D0S143E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens cytochrome P450, subfamily XIB, polypeptide 1
(steroid 11-beta-hydroxylase)
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M85450; EST: EST01966; Primer: M13 Forward;
THC id: THC91329
DNA Seq. Acc.: T04959; EST: EST02847; Primer: M13-21; THC
id: THC91329
DNA Seq. Acc.: T04960; EST: EST02848; Primer: M13 Reverse
Insert lengths(kb): 1.799999952316284
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) [CYP11B]
Insert Size (kb)
1.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8; PstI--4.8; HindIII--4.8.
EST01966 matches GenBank accession HUMCYPXIB with 67.35% identity over the sequence range: 113-256.
EST01966 contains a Line-1 repeat element.
This sequence record is for human cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) (CYP11B1).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

J C Venter, personal communication

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