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Neospora caninum Dubey et al.
Neospora caninum Dubey et al.
規格:
價格:
編號:B221925
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Neospora caninum Dubey et al.
商品貨號
B221925
Strain Designations
NC-Illinois
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

培養基
Isolation
clinical specimen - animal
Illinois, United States
Isolation date: October 27, 1999
Product Format
frozen
Storage Conditions
liquid nitrogen vapor phase
Type Strain
no
Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 95%; horse serum, 5%
Growth Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Duration: The organism is propagated in Vero cells.
Protocol:
  1. Scrape the Vero cells into the culture medium when holes develop in the monolayer (tachyzoite clusters are observed in the cells and 7 micrometer long banana-shaped tachyzoites are free in the medium).
  2. Transfer approximately 0.5 ml of the harvested medium (or approximately 500,000 tachyzoites) to another T25 flask containing a fresh monolayer of vero cells.
  3. Incubate at 37C.
Interval: approximately 1 week
Subcultivation
Protocol:
  1. Scrape the Vero cells into the culture medium when holes develop in the monolayer (tachyzoite clusters are observed in the cells and 7 micrometer long banana-shaped tachyzoites are free in the medium).
  2. Transfer approximately 0.5 ml of the harvested medium (or approximately 500,000 tachyzoites) to another T25 flask containing a fresh monolayer of vero cells.
  3. Incubate at 37C.
Interval: approximately 1 week
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor
MM McAllister
Special Collection
NSF - Protistology
Year of Origin
October 27, 1999
References

Barber JS, et al. Characterization of the first European isolate of Neospora caninum (Dubey, Carpenter, Speer, Topper and Uggla). Parasitology 111: 563-568, 1995. PubMed: 8559588

Gondim LF, et al. Variation of the internal transcribed spacer 1 sequence within individual strains and among different strains of Neospora caninum. J. Parasitol. 90: 119-122, 2004. PubMed: 15040677

Holstein dairy calf (Bos taurus)

McAllister MM, et al. A probable source of Neospora caninum infection in an abortion outbreak in dairy cows. Bovine Practitioner 39: 69-74, 2005.

Cross References

Nucleotide (GenBank) : AY259041 Neospora caninum strain NC-Illinois internal transcribed spacer 1, complete sequence

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