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Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Genetic Target: Preparation includes fragments from the 5'UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3' UTR region of the West Nile Virus genome. The length of the nucleic acid is approximately 1500 bp.
Length: ~1500 bp
Format Provided: Dried RNA
Starting Amount: 1.0 x 108 Copy Numbers (CN)
Downstream compatibility: Reverse Transcription PCR or other molecular applications
Rondini S, et al. Development of multiplex PCR-ligase detection reaction assay for detection of West Nile virus. J Clin Microbiol 46(7): 2269-2279, 2008. PubMed: 18495862
Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368
Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934
Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613
Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069
Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997
Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762
Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039
Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227
