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pWL625Amy & pBR325Amy
pWL625Amy & pBR325Amy
規(guī)格:
價格:
編號:B222100
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pWL625Amy & pBR325Amy
商品貨號
B222100
Designations
pWL625Amy & pBR325Amy
Species
Geobacillus stearothermophilus (Donk) Nazina et al.
培養(yǎng)基
Depositors
CPC International, Inc.
Applications
The Escherichia coli host contains two plasmids: pWL6225 and pBR325 (ATCC 31792).
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR
Cross references:
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
Insert lengths(kb): 5.400000095367432
Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A)
Insert Size (kb)
5.400
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The total size of the clone is 20.4 kb in pWL625 or 11.2 kb in pBR325.
The Escherichia coli host contains two plasmids: pWL6225 and pBR325 (ATCC 31792).
References

Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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