| 產品名稱 |
p158 |
| 商品貨號 |
B222149 |
| Designations |
p158 |
| GenBank Number |
S53290
|
| Species |
Mus musculus, mouse |
| Depositors |
JG Granneman |
| Vector |
Construct size (kb): 3.180000066757202 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.1800000071525574 Tissue: white adipose Gene product: adrenergic, beta-3-, receptor [Adrb3] |
| Insert Size (kb) |
0.180 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.2; EcoRI--3.2; HindIII--3.2; XhoI--3.2; BamHI/EcoRI--3.2, 0.215. Partial cDNA clone derived from a region containing an intron in genomic DNA. Constructed after amplification from cDNA with these primers: 5'-GGACTTTCGCGACGCCT-3'; 5'-GCATCCATGGACGTTGCTTGTC-3'. The product was shortened to 180 bp before cloning. Transcription from the SP6 promoter gives the sense strand. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Granneman JG, et al. Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block. Mol. Pharmacol. 42: 964-970, 1992. PubMed: 1336117
James G Granneman, personal communication
|