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Hp2010
Hp2010
規格:
價格:
編號:B222154
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hp2010
商品貨號
B222154
Designations
Hp2010
GenBank Number

Y00724

培養基
Species
Homo sapiens, human
Depositors
H Kuivaniemi
Vector
Construct size (kb): 7.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SphI
Vector end: SphI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL1A2
Genomic copy number: unique
Gene name: collagen, type I, alpha 2
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q21.3-q22.1
Tissue: placenta
Type of DNA: cDNA
Insert end: SphI linkers
Insert end: SphI linkers
Insert size (kb): 4.6
Cross references: DNA Seq. Acc.: Y00724
Nucleotides 1-2372 of the insert correspond to
nucleotides 1-2372 of Y00724.
Insert lengths(kb): 4.599999904632568
Tissue: placenta
Gene product: collagen, type I, alpha 2 [COL1A2]
Alleles: A1, B1, K1, C1, A2, C2, K2, b2
Insert Size (kb)
4.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.9; NcoI--5.9, 1.4; PstI--4.4, 2.4, 0.48; SphI--4.6, 2.7; XhoI-- 7.3.
Insert contains the following restriction sites (approximate kb from the 5' end): AvaI--1.7, 2.3; EcoRI--4.3; NcoI--1.8, 3.3; PstI--0.45, 3.05; PvuII--0.4, 0.5, 0.8, 1.1, 1.5, 2.4; RsaI--2.7, 3.4; XhoI--2.8.
This insert contains a PvuII site in exon 25 (nt 1521) that has been shown to be polymorphic.
References

Constantinou CD, et al. A HaeIII polymorphism at the D10S101 locus. Nucleic Acids Res. 18: 5577, 1990. PubMed: 1977119

Kuivaniemi H, et al. Structure of a full-length cDNA clone for the prepro alpha2(I) chain of human type I procollagen. Biochem. J. 252: 633-640, 1988. PubMed: 3421913

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